Fields specific to MST
schema_version
Description: The schema version used to annotate the MST method specific parameters
Required: yes
Path: metadata.method_specific_parameters.schema_version
Type: enumerator (string)
Options: ('0.9.10')
experiment_type
Description: The type of physical parameter that was sought
Required: yes
Path: metadata.method_specific_parameters.experiment_type
Type: enumerator (string)
Options: ('Affinity','Concentration', 'Other')
signal_type
Description: The type of signal that was being measured
Required: yes
Path: metadata.method_specific_parameters.signal_type
Type: enumerator (string)
Options: ('Initial intensity', 'TRIC/MST', 'Spectral shift')
excitation_led_color
Description: The color of the excitation LED used for the experiment.
NOTE that colors are specific to the combination in which it occurs,
e.g. the GREEN in a BLUE/GREEN instrument, is not the same as the GREEN in a GREEN/RED instrument
Required: yes
Path: metadata.method_specific_parameters.excitation_led_color
Type: enumerator (string)
Options: (
'RED (ex 605-645nm, em 660-720nm)',
'RED (ex 610-645nm, em 680-720nm)',
'GREEN (ex 555-585nm, em 605-690nm)',
'GREEN (ex 515-550nm, em 565-600nm)',
'BLUE (ex 480-500nm, em 515-550nm)',
'BLUE (ex 460-500nm, em 515-560nm)',
'UV (ex 260-300nm, em 330-380nm)',
'Spectral shift')
excitation_led_power
Description:The power, in percent, of the excitation LED used in experiment
Required: yes
Path: metadata.method_specific_parameters.excitation_led_power
Type: number (float)
Min: 0
Max: 100
ir_mst_laser_power
Description:The power of the infrared LASER used in the experiment
in percentages. Even though a change in nomenclature occurred in
Nanotemper's control software, the underlying data is still stored in
percentages. Use the following conversion;
Low = 20, Medium = 40, High = 60
Required: yes
Path: metadata.method_specific_parameters.ir_mst_laser_power
Type: number (float)
Min: 0
Max: 100
temperature
Description:The power of the infrared LASER used in the experiment
in percentages. Even though a change in nomenclature occurred in
Nanotemper's control software, the underlying data is still stored in
percentages. Use the following conversion;
Low = 20, Medium = 40, High = 60
Required: yes
Path: metadata.method_specific_parameters.temperature
Type: object (Temperature)
Contains: see Temperature
measurements
Description:List of the information about each
measurement. This includes target(s),
ligand(s), chemical environment, and the
position of the sample within the instrument
Required: yes
Path: metadata.method_specific_parameters.measurements
Type: array of object (Measurement)
Contains: see Measurement
data_analysis
Description:The details of how data analysis was performed to obtain results
Required: yes
Path: metadata.method_specific_parameters.data_analysis
Type: array of object (MST_data_analysis)
Contains: see MST_data_analysis